Anti-arthritic activity of methanol
extract of Cyathocline purpurea
(whole plant) in Freund’s complete adjuvant-induced arthritis in rats
Abstract
Aim of the study:
To investigate anti-arthritic activity of methanolic extract of Cyathocline purpurea (MECP) in Freund’s complete
adjuvant (FCA)-induced arthritis in rats.
Methods:
The MECP was prepared and subjected to acute oral toxicity in mice and tested against
FCA induced arthritis in rats. Arthritis assessment was done by measuring–paw volume,
joint diameter, pain threshold, thermal hyperalgesia, mechanical nociceptive threshold
and body weight. Haematological, serum, biochemical and in-vivo anti-oxidant parameters
were measured on the last day of the study. Histopathological and radiological analyses
of ankle joints were also done. MECP was administered at the dose of 100, 200 and
400 mg/kg body weight.
Results:
MECP dose dependently showed anti-arthritic activity which was evident with decrease
in paw volume, joint diameter and increase in pain threshold, paw withdrawal latency,
mechanical nociceptive threshold and body weight when compared to arthritic control
group. MECP (400 and 200 mg/kg) exhibits significant (P<0.001 and P<0.01,
respectively) anti-arthritic activity by increasing levels of RBC, Hb and by
decreasing levels of WBC, platelets and also serum C- reactive protein (CRP) and
Rheumatoid factor (RF). The anti-arthritic activity was also confirmed with the
altered biochemical parameters (AST, ALT, ALP and total protein level) and anti-oxidant
parameters (SOD, MDA and GSH). MECP (400 and 200 mg/kg) and diclofenac (10mg/kg)
also inhibited joint destruction (Histopathological and radiological analysis).
Conclusion:
C. purpurea may be a potential preventive
or therapeutic candidate for the treatment of inflammation and arthritis.